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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
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Prediction of protein-protein interactions: the CAPRI experiment, its evaluation and implications

Shoshana J Wodak1, Raúl Méndez

  • 1Service de Conformation de macromolecules Biologique et Bioinformatique, Centre de Biologie Structurale et Bioinformatique, Université Libre de Bruxelles, CP 263, Boulevard du Triomphe, 1050 Bruxelles, Belgium. shosh@ucmb.ulb.ac.be

Current Opinion in Structural Biology
|April 20, 2004
PubMed

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Ligand Binding Sites

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Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
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