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Prolonged bleeding time of Chediak-Higashi cats corrected by platelet transfusion
B E Cowles1, K M Meyers, K J Wardrop
1Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman 99164-6520.
Insights
Cats with Chediak-Higashi syndrome (CHS) experience prolonged bleeding due to platelet storage pool deficiency (SPD). Platelet transfusions effectively corrected bleeding times in this animal model, indicating its utility for studying platelet hemostasis.
Area of Science:
- Veterinary Hematology
- Animal Models of Disease
- Platelet Physiology
Background:
- Chediak-Higashi syndrome (CHS) in cats is characterized by a platelet storage pool deficiency (SPD).
- SPD leads to prolonged bleeding times, impacting hemostasis.
- Understanding the efficacy of platelet transfusions is crucial for managing bleeding disorders.
Purpose of the Study:
- To evaluate the effectiveness of platelet transfusions in correcting prolonged bleeding times in cats with CHS.
- To establish CHS cats as a viable animal model for studying platelet storage pool deficiency.
- To investigate the relationship between donor platelet count and bleeding time in CHS cats.
Main Methods:
- Ten cats with CHS were transfused with 51Cr-labeled platelets from normal donors.
- Bleeding times were measured before and after transfusion.
- Donor platelet counts were correlated with bleeding times.
Main Results:
- Post-transfusion donor platelet counts ranged from 40,000-60,000/microliter.
- Bleeding times significantly decreased from a baseline of 9.1 min to 1.7 min when platelet counts reached 50,000/microliter.
- A strong inverse correlation (r = -0.92) was observed between donor platelet count and bleeding time.
Conclusions:
- Platelet transfusion effectively corrects prolonged bleeding times in CHS cats, an animal model of platelet SPD.
- CHS cats serve as an appropriate model for evaluating the hemostatic capabilities of transfused platelets.
- The study confirms a platelet abnormality as the cause of prolonged bleeding in CHS cats.
Abstract:
Cats with the Chediak-Higashi syndrome (CHS) have a platelet storage pool deficiency (SPD). Ten CHS cats were transfused with a concentrate of 51Cr-labeled platelets prepared from normal donor cats. One hour after transfusion, the donor platelet count in CHS recipient cats was 40,000-60,000/microliters. Bleeding time before transfusion was 9.1 +/- 3.0 min. When donor platelet count in CHS cats was 50,000/microliters, bleeding time was 1.7 +/- 0.2 min. Bleeding time of normal cats was 1.4 +/- 0.3 min. Bleeding time increased to 3.3 +/- 0.2 min and to 5.3 +/- 0.2 min when the platelet count was 30,000/microliters, and 15,000/microliters, respectively. The close inverse relationship between bleeding time and number of donor platelets in CHS cats (r = -0.92), suggests that prolonged bleeding time is due to a platelet abnormality, that platelet transfusion can effectively correct prolonged bleeding time in an animal model of platelet SPD and that CHS cats may be an appropriate animal model to evaluate hemostatic capabilities of transfused platelets.