Related Experiment Videos
DISC1 localizes to the centrosome by binding to kendrin
Ko Miyoshi1, Masato Asanuma, Ikuko Miyazaki
1Department of Brain Science, Graduate School of Medicine and Dentistry, Okayama University, 2-5-1 Shikatacho, Okayama 700-8558, Japan. miyoshi@cc.okayama-u.ac.jp
Biochemical and Biophysical Research Communications
|April 20, 2004
Summary
Disrupted-In-Schizophrenia 1 (DISC1) protein interacts with kendrin, localizing DISC1 to the centrosome. This centrosomal localization may link DISC1 to the pathophysiology of mental disorders.
Area of Science:
- Neuroscience
- Cell Biology
- Genetics
Background:
- Disrupted-In-Schizophrenia 1 (DISC1) is a gene implicated in major mental disorders.
- DISC1 is disrupted by a specific chromosomal translocation.
- The function and localization of DISC1 are not fully understood.
Purpose of the Study:
- To identify proteins that interact with DISC1.
- To determine the cellular localization of DISC1.
- To investigate the role of DISC1 in centrosomal function and mental disorder pathophysiology.
Main Methods:
- Yeast two-hybrid screening of a human brain cDNA library.
- Immunoprecipitation assays in mammalian cells.
- Immunocytochemical analysis for colocalization studies.
Main Results:
- DISC1 interacts with kendrin/pericentrin-B, a centrosome-localized protein.
- The interaction involves specific residues (446-533) of DISC1.
- DISC1 and kendrin colocalize to the centrosome in mammalian cells.
Conclusions:
- DISC1 localizes to the centrosome through binding with kendrin.
- Kendrin anchors the gamma-tubulin complex at the centrosome, influencing microtubule nucleation.
- DISC1's centrosomal localization suggests a role in mental disorder pathophysiology via centrosomal function.