Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The never-ending story of peptide O-xylosyltransferase.

I B H Wilson1

  • 1Institut für Chemie der Universität für Bodenkultur, Muthgasse 18, 1190 Wien, Austria. iain.wilson@boku.ac.at

Cellular and Molecular Life Sciences : CMLS
|April 20, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of an α-l-fucosidase from the periodontal pathogen Tannerella forsythia.

Virulence·2015
Same author

Expression, purification and preliminary crystallographic analysis of Drosophila melanogaster lysosomal α-mannosidase.

Acta crystallographica. Section F, Structural biology and crystallization communications·2012
See all related articles

Peptide O-xylosyltransferase initiates glycosaminoglycan synthesis, crucial for proteoglycan development. Its study reveals evolutionary insights and links mutations to developmental defects in model organisms.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Developmental Biology

Background:

  • Peptide O-xylosyltransferase (EC 2.4.2.26) catalyzes the initial step in chondroitin, dermatan, and heparan sulfate biosynthesis.
  • This enzyme is essential for proteoglycan core protein modification in the endoplasmic reticulum and/or cis-Golgi.
  • It belongs to glycosyltransferase family 14, sharing homology with enzymes involved in O-glycan branching.

Purpose of the Study:

  • To review the historical progression and current understanding of peptide O-xylosyltransferase.
  • To explore the genetic basis and evolutionary conservation of this enzyme across species.
  • To highlight the functional significance of glycosaminoglycans in animal development.

Main Methods:

  • Purification and cDNA cloning of peptide O-xylosyltransferase.

Related Experiment Videos

  • Analysis of primary protein structure and gene homology.
  • Investigation of mutant phenotypes in model organisms (e.g., Caenorhabditis elegans).

    • Main Results:

    • Vertebrates possess two related genes for xylosyltransferase, with only the first isoform showing detectable enzymatic activity in recombinant form.
    • Caenorhabditis and Drosophila each have a single gene for this enzyme.
    • A mutation in the Caenorhabditis xylosyltransferase gene (sqv-6 mutant) results in defective vulval morphogenesis.

    Conclusions:

    • Peptide O-xylosyltransferase plays a critical role in animal development through its involvement in glycosaminoglycan chain synthesis.
    • The enzyme's evolutionary conservation suggests fundamental biological importance.
    • Further research is needed to elucidate intracellular localization and structure-function relationships.