Related Experiment Video
Updated: Aug 24, 2026

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
Published on: January 30, 2021
Polysaccharides from Hibiscus sabdariffa flowers stimulate proliferation and differentiation of human keratinocytes
C Brunold1, A Deters, F Knoepfel-Sidler
1University of Applied Science Wädenswil, Pharmaceutical Biotechnology, Wädenswil, Switzerland.
Abstract:
Raw polysaccharides, previously described in detail, were isolated from the flowers of Hibiscus sabdariffa L. and fractionated by ion exchange chromatography into one neutral and three acidic subfractions. Raw polysaccharides and all acidic subfractions caused a strong induction of proliferation of human keratinocytes (HaCaT) of up to 40 %, while the neutral polymers were ineffective. While mitochondrial activity was not influenced, raw polysaccharides induced early differentiation of primary natural human keratinocytes, as determined by involucrin formation.
Related Concept Videos
Multipotency and Niche of Bulge Stem Cell
iPS Cell Differentiation
Renewal of Skin Epidermal Stem Cells

