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Updated: Jul 31, 2026
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
16S to 23S rDNA spacer fragment length polymorphism of Aeromonas sp
Marzena Laganowska1, Adam Kaznowski
1Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, Fredry 10, 61-701 Poznań, Poland.
Abstract:
We analyzed polymorphism of the PCR-amplified 16S-23S rDNA spacer of Aeromonas species. A total of 69 isolates representing 18 DNA hybridization groups were used in this study. The analysis of PCR products of 16S-23S rDNA spacers revealed patterns consisting of two to eight DNA fragments. The fragment sizes ranged from 730 to 1050 bp. DNA patterns revealed a considerable genetic diversity between species and within a species. When a procedure to eliminate heteroduplex formation was performed, the number of bands was reduced to 2-5. Nevertheless the homoduplex ISR (intergenic spacer region) patterns obtained were not useful for species distinguishing.
