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Bubble cycling and standing waves in ultrasonic cell lysis
1Department of Biophysics, School of Medicine and Dentistry, University of Rochester, NY 14642.
Ultrasound in Medicine & Biology
|January 1, 1992
Summary
Bubble cycling, not trapped bubbles, primarily causes ultrasonic cell lysis in rotating vessels. Trapped bubbles contribute significantly under specific conditions, revealing mechanisms of ultrasound-induced cell death.
Area of Science:
- Biophysics
- Acoustics
- Cell Biology
Background:
- Two mechanisms explain ultrasonic cell lysis dependence on rotation: trapped bubbles in standing waves and bubble recycling.
- Bubble recycling is currently the favored explanation for ultrasound-induced cell lysis.
Purpose of the Study:
- To differentiate and quantify the contributions of trapped bubbles versus bubble recycling to cell lysis.
- To investigate the role of standing waves and bubble recycling in ultrasound-mediated cell disruption.
Main Methods:
- Murine P388 cells were exposed to 1.07 MHz continuous-wave ultrasound.
- Experiments varied exposure vessel composition and configuration to manipulate standing waves and bubble recycling.
- Ultrasound intensity was maintained at 5 W/cm2 (ISP) for 5 minutes.
Main Results:
- Bubble cycling was identified as the predominant mechanism for cell lysis in rotating vessels.
- Trapped bubbles in standing waves also contributed significantly to cell lysis under certain experimental conditions.
- Results suggest a combined effect of bubble cycling and trapped bubbles in ultrasonic cell lysis.
Conclusions:
- Bubble cycling is the primary driver of cell lysis during rotating vessel ultrasound exposure.
- Standing wave effects and trapped bubbles play a secondary but significant role in specific scenarios.
- Understanding these mechanisms is crucial for optimizing ultrasound-based cell processing techniques.