Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nuclear RNP complex assembly initiates cytoplasmic RNA localization.

Tracy L Kress1, Young J Yoon, Kimberly L Mowry

  • 1Box G-J2, Brown University, Providence, RI 02912, USA.

The Journal of Cell Biology
|April 21, 2004
PubMed
Summary

Cytoplasmic mRNA localization, crucial for cell polarity and development, begins in the nucleus. Specific RNA-binding proteins recognize and target mRNAs for transport and localization within the cell.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Secretome translation shaped by lysosomes and lunapark-marked ER junctions.

Nature·2025
Same author

Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates in Xenopus oocytes.

Scientific reports·2024
Same author

Intrinsically disordered regions and RNA binding domains contribute to protein enrichment in biomolecular condensates in <i>Xenopus</i> oocytes.

bioRxiv : the preprint server for biology·2023
Same author

Phosphorylation alters FMRP granules and determines their transport or protein synthesis abilities.

bioRxiv : the preprint server for biology·2023
Same author

Lysosomal release of amino acids at ER three-way junctions regulates transmembrane and secretory protein mRNA translation.

bioRxiv : the preprint server for biology·2023
Same author

Hitting the mark: Localization of mRNA and biomolecular condensates in health and disease.

Wiley interdisciplinary reviews. RNA·2023

Area of Science:

  • Developmental Biology
  • Molecular Cell Biology
  • Genetics

Background:

  • Cytoplasmic mRNA localization is essential for establishing cell polarity and patterning during embryonic development.
  • In Xenopus oocytes, maternal mRNA localization relies on specific RNA sequences recognized by protein factors.
  • The precise timing and mechanism of RNA-protein interactions in mRNA transport remain unclear.

Purpose of the Study:

  • To investigate the temporal and spatial dynamics of RNA-protein interactions during mRNA localization.
  • To identify the key protein factors involved in the early stages of RNA transport and localization.
  • To elucidate how RNP complexes are remodeled from the nucleus to the cytoplasm.

Main Methods:

  • Analysis of ribonucleoprotein (RNP) complexes isolated from both nuclear and cytoplasmic compartments.

Related Experiment Videos

  • Characterization of RNA-binding proteins associated with localized maternal mRNAs.
  • Comparative analysis of RNP composition during different stages of transport.

    • Main Results:

    • Specific RNA-binding proteins associate with localized RNAs within the nucleus, marking an early step in the localization pathway.
    • RNP complexes undergo remodeling upon transport into the cytoplasm, with recruitment of additional factors.
    • Evidence suggests that nuclear RNP complex formation is critical for targeting RNAs to their cytoplasmic destinations.

    Conclusions:

    • Cytoplasmic RNA localization is initiated in the nucleus through the binding of specific RNA-binding proteins.
    • Nuclear RNP complex assembly serves as a crucial targeting mechanism for subsequent cytoplasmic localization.
    • Understanding these early nuclear events provides insight into the regulation of mRNA transport and cell patterning.