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Related Experiment Videos

Scanning protocol choice affects optical coherence tomography (OCT-3) measurements.

Samiah Zafar1, Rabia Gurses-Ozden, Manchima Makornwattana

  • 1Department of Ophthalmology, The New York Eye and Ear Infirmary, New York, New York, USA.

Journal of Glaucoma
|April 21, 2004
PubMed
Summary

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The optimal sampling density for OCT-3 assessment of parapapillary retinal nerve fiber layer (RNFL) thickness is 256 axial determinations. Consistent scanning protocols are crucial for serial evaluations in glaucoma patients.

Area of Science:

  • Ophthalmology
  • Medical Imaging
  • Glaucoma Research

Background:

  • Optical Coherence Tomography (OCT) is vital for diagnosing and monitoring glaucoma.
  • Accurate measurement of parapapillary retinal nerve fiber layer (RNFL) thickness is essential for glaucoma assessment.
  • Different OCT scanning protocols may influence RNFL thickness measurements.

Purpose of the Study:

  • To determine the optimal sampling density for OCT-3 assessment of parapapillary RNFL thickness.
  • To compare RNFL measurements obtained using different OCT-3 scanning protocols (fast, regular, and customized).

Main Methods:

  • Enrolled normal subjects, ocular hypertensive patients, and open-angle glaucoma patients.
  • Utilized "fast" (256 A-scans/1.92s) and "regular" (512 A-scans/1.28s per scan, total 3.84s) RNFL scan protocols.

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  • Created a customized 256-point scan from the regular scan and calculated mean RNFL thickness and coefficients of variation (CV).

    • Main Results:

    • No significant differences in RNFL thickness or CV were found between scanning protocols in eyes with visual field defects.
    • In normal eyes, RNFL thickness was significantly greater with the fast scan (P=0.01), but CV showed no difference across protocols.
    • All tested protocols demonstrated good reproducibility.

    Conclusions:

    • Optimal sampling density for OCT-3 parapapillary RNFL thickness assessment is 256 axial determinations.
    • While scanning protocols show good reproducibility, maintaining a consistent protocol is critical for serial OCT-3 evaluations.
    • The findings suggest that 256 axial determinations are sufficient for reliable RNFL assessment.