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[Study on the neurotoxicity and brain tissue HSPs level in benzo[a]pyrene exposed mice]
Baijie Tu1, Tangchun Wu, Hanzhen He
1Department of Occupational Medicine, Chongqing University of Medical Science, Chongqing 400016, China.
Objective:
To study the neurotoxicity and brain tissue HSP70, HSP90 beta level in benzo [a]pyrene exposed mice.
Methods:
50 Kunming mice were divided into 5 groups randomly, the exposed groups(3 dose level groups), the vehicle control group and the standard control group, every group get 10 mice. The exposed groups were administrated by intraperitoneal injection with BaP dissolved in vegetable oil at 7.8, 3.2 and 1.3 mg/kg BW once, 4 times/week specially, the vehicle control group was given vegetable oil parallel and the standard control group was not given additional treatment. Observing general conditions and nerve symptoms of the animals. After 8 weeks experiments, the mice were killed and the brains were removed. Detect the ratio of brain-weight/body-weight and the Hsp70 and Hsp90 beta level of the mice brain homogenate by the method of western blot.
Results:
1. In the high and medium dose groups, the brain-weight was lower than that of control groups. In the high dose group, the ratio of brain-weight/body-weight was lower than that of other groups. 2. In the low dose BaP group, HSP70 level were much higher than that of control groups. In the medium dose BaP group and high dose BaP group, HSP90 beta level were higher than that of control groups.
Conclusion:
BaP can damage the never system of the mice; the high the dose was, the high the HSPs90 level were.
