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[Synergetic germicidal effect of ultrasonic with glutaraldehyde and its application in clinical disinfection]
1Shool of Public Health, West China Medical Center of Sichuan University, Chengdu 610041, China.
Objective:
To study the synergetic germicidal effect of ulstrasonic with glutaraldehyde and the feasibility of combining ulstrasonic and glutaraldehyde to disinfect endocopes and medical instruments.
Methods:
We determined the respective and the synergetic killing rates of Bacillus subtilis var. niger of ulstrasonic and glutaraldehyde using quantitative suspenion germicidal tests and simulated on-the-spot tests.
Results:
In suspenion germicidal tests, because of the synergetic germicidal effect, the concentration of glutaraldehyde needed for sterilize spores was decreased from 20 mg/ml to 5 mg/ml for the same killing time(4 h), and when the concentrations were still 20 mg/ml, its killing time was shorten from 4 h to 1 h. Further more, the solutions containing 5 mg/ml glutaraldehyde could sterilize spores of Bacillus subtilis var. niger in 2 h. In the simulated on-the-spot tests, the concentration of glutaraldehyde needed for sterilization was decreased from 20 mg/ml to 5 mg/ml and its killing time was shorten from 4 h to 1 h under the synergetic germicidal effect.
Conclusion:
It indicats the feasibility of combining ulstrasonic and glutaraldehyde to disinfect instruments in clinic.
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