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Updated: Aug 11, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
[Determination of protein allergenicity--BN rat model]
Xudong Jia1, Ning Li, Wei Wang
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Objective:
Protein allergenicity was investigated in BN rat model.
Methods:
OVA or BSA was administered i.p. (1 mg/ml) or orally (10 mg/ml) to male BN rats for 6 weeks. In different intervals, blood samples were obtained from the orbital plexus for PCA and histamine determination. In addition, blood pressure of rats in OVA-treated group and control group was measured.
Results:
Relatively high titres (1/32-1/128) IgE responses were provoked by administration of OVA, while BSA stimulated only low titres (1/2-1/4) IgE. Compared with the controls, the plasma histamine concentrations of OVA groups were higher. An oral challenge with OVA did not induce a clear effect on blood pressure in the majority of rats. However, two rats demonstrated a temporary drop in blood pressure.
Conclusion:
BN rat model may be a suitable animal model to determine protein allergenicity.
