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Updated: Aug 17, 2026

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells]
Shisong Fang1, Tao Liu, Pingjian Deng
1South China University of Technology, Guangzhou 510642, China.
Objective:
Cecropin D gene was cloned into methanol nutritional vector-pPICZ alpha-A, linked with alpha-factor signal peptide.
Methods:
Positive recombinant was gotten through PCR and EcoR I restriction enzyme. The positive recombinant vector was transformed into Pichia pastoris yeast strain GS115.
Results:
The positive recombinant yeast velum was fermented and its expressive product was assayed by the method of anti-germ.
Conclusion:
Cecropin D gene was cloned into yeast cell and its expressive products has high activity of killing bacteria, Which could be developed into a new type of anti-bacterial medicine.
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