In vivo genotoxicity of atrazine to anuran larvae

Atrazine has been an environmental contaminant for more than two decades. While there can be little dispute as to the presence of atrazine in non-target watersheds, the debate has centered on the consequences of this contamination. The purpose of this study was to determine if atrazine is genotoxic to developing anurans. Anurans are one of the groups that have the highest potential for being affected by watershed contamination. In initial studies, larvae from two anuran species were exposed to known genotoxic agents. Upon flow cytometric analysis, those organisms exposed to the genotoxic agents resulted in a statistically significant increase in nuclear heterogeneity. Having demonstrated that flow cytometric analysis could be used to detect genotoxicity in anuran larvae, the larvae of the two species were exposed to different levels of atrazine for various durations. The concentrations and lengths of exposure were consistent (albeit on the higher side) with conditions found in the Midwestern US. In neither species was an increase in nuclear heterogeneity observed. Thus, atrazine at levels and time of exposure representing conditions found contaminating Midwestern watersheds does not appear to be genotoxic to developing anurans.