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Related Experiment Videos

Enteric nematodes induce stereotypic STAT6-dependent alterations in intestinal epithelial cell function.

Kathleen B Madden1, Karla Au Yeung, Aiping Zhao

  • 1Department of Pediatrics, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|April 22, 2004
PubMed
Summary

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Gastrointestinal nematode infections alter gut function via STAT6-dependent pathways. These changes affect epithelial cells directly and indirectly through enteric nerves, impacting host physiology.

Area of Science:

  • Immunology
  • Gastroenterology
  • Parasitology

Background:

  • Gastrointestinal nematode infections significantly impact host immune and physiological responses.
  • Previous work showed T-helper 2 (Th2) cytokines interleukin-4 (IL-4) and IL-13 induce Signal Transducer and Activator of Transcription 6 (STAT6)-dependent changes in intestinal epithelial cells.

Purpose of the Study:

  • To determine if nematode-induced effects on intestinal epithelial cell function are generic across different nematode species.
  • To investigate the dependence of these effects on STAT6.
  • To elucidate whether the effects are direct on epithelial cells or mediated by enteric nerves.

Main Methods:

  • Infection of BALB/c mice with three distinct gastrointestinal nematodes: Heligmosomoides polygyrus, Nippostrongylus brasiliensis, and Trichinella spiralis.

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  • Assessment of intestinal epithelial cell function, including resistance, glucose absorption, and secretory responses.
  • Evaluation of the role of STAT6 and enteric nerves in mediating these functional changes.

    • Main Results:

    • Infection with any of the three nematodes altered intestinal epithelial cell function.
    • Observed alterations included decreased resistance, reduced glucose absorption, and diminished secretory responses to 5-hydroxytryptamine and acetylcholine.
    • These nematode-induced modifications were STAT6-dependent and resulted from both direct epithelial cell effects and indirect effects mediated via enteric nerves.

    Conclusions:

    • Gastrointestinal nematode infections induce generic, STAT6-dependent alterations in intestinal epithelial cell function.
    • These functional changes are mediated by both direct effects on epithelial cells and indirect pathways involving enteric nerves.
    • The findings highlight the complex interplay between parasitic infection, host immunity, and enteric nervous system regulation of gut physiology.