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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
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  1. Home
  3. Detection Of Mouse And Rat Urinary Aeroallergens With An Improved Elisa.
  1. Home
  3. Detection Of Mouse And Rat Urinary Aeroallergens With An Improved Elisa.

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Detection of mouse and rat urinary aeroallergens with an improved ELISA.

Anne Korpi1, Rauno Mäntyjärvi, Jaakko Rautiainen

  • 1Department of Environmental Sciences, Researech and Development Unit of Environmental Health, University of Kuopio, Bioteknia 2, PO Box 1627, 70211 Kuopio, Finland.

The Journal of Allergy and Clinical Immunology
|April 22, 2004

View abstract on PubMed

Summary
This summary is machine-generated.

Sensitive ELISA methods were developed to detect low levels of mouse (Mus m 1) and rat (Rat n 1) aeroallergens in animal facilities. These enhanced assays improve risk assessment for laboratory animal work.

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Area of Science:

  • Occupational Health
  • Environmental Science
  • Immunology

Background:

  • Risk analysis in laboratory animal work necessitates sensitive allergen monitoring.
  • Commercial ELISA kits exist for mouse (Mus m 1) and rat (Rat n 1) allergens but require enhanced sensitivity for low airborne levels.

Purpose of the Study:

  • To enhance ELISA kit sensitivity for detecting aeroallergens below 1 ng/m³.
  • To validate these enhanced methods using air samples from a laboratory animal facility.

Main Methods:

  • Collection of personal and stationary air samples within an animal facility during various laboratory animal work tasks.
  • Optimization of ELISA assay parameters and reagent selection to improve sensitivity.

Main Results:

  • Achieved detection limits of 0.1 ng/m³ for Mus m 1 and 0.8 ng/m³ for Rat n 1.
  • Successfully detected mouse and rat aeroallergens in areas without direct animal presence or dirty cages.
  • Demonstrated improved sensitivity of ELISA assays through careful parameter and reagent selection.

Conclusions:

  • The developed sensitive assays aid in the risk assessment of laboratory animal work.
  • Standardized analytical procedures and occupational exposure limits for laboratory animal allergens are still lacking.