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Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter.

A Wolff1, I R Perch-Nielsen, U D Larsen

  • 1Mikroelektronik Centret (MIC), Technical University of Denmark (DTU), Orsteds Plads, DTU Bldg 345east, DK-2800, Lyngby, Denmark. aw@mic.dtu.dk

Lab on a Chip
|April 22, 2004
PubMed
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This study introduces a microfluidic cell sorter chip that integrates sample handling, focusing, and detection. The device efficiently sorts fluorescent beads from cells at high throughput, advancing integrated micro-analysis systems.

Area of Science:

  • Microfluidics
  • Biotechnology
  • Analytical Chemistry

Background:

  • Microfabricated devices offer potential for integrated analysis systems.
  • Existing cell sorting technologies can be complex and prone to cell loss.

Purpose of the Study:

  • To develop a novel, pressure-driven microfluidic cell sorter chip with advanced functional integration.
  • To demonstrate the chip's capability for high-throughput cell sorting and analysis.

Main Methods:

  • Fabrication of a microfluidic chip with integrated hydrodynamic focusing, cell sorting, and cell culture capabilities.
  • Utilizing fluorescent latex beads and chicken red blood cells for sorting experiments.
  • Incorporating integrated optics for real-time cell detection.

Related Experiment Videos

Main Results:

  • Successful sorting of fluorescent latex beads from chicken red blood cells.
  • Achieved substantial cell enrichment at a high sample throughput of 12,000 cells/second.
  • Demonstrated the functionality of integrated components including hydrodynamic focusing and cell culture chambers.

Conclusions:

  • The developed microfluidic cell sorter chip offers a highly integrated solution for cell analysis.
  • The design minimizes cell loss and enables efficient sorting and culturing on-chip.
  • This work contributes to the development of fully integrated micro-scale cell sorting and analysis systems.