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Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor
Yun-mei Yang1, Hong-cui Cao, Zhe-rong Xu
1First Affiliated Hospital, College of Medical, Zhejiang University, Hangzhou 310003, China.
Objective:
To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease.
Methods:
To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC).
Results:
rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease.
Conclusion:
Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.
