Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Lipid peroxidation in isolated rat nephron segments.

H Ha¹, H Endou

¹Department of Pharmacology, Faculty of Medicine, University of Tokyo, Japan.

The American Journal of Physiology

|August 1, 1992

Summary

This study developed a microassay to measure lipid peroxides (LPO) in kidney tissues. Results show proximal tubules have higher LPO levels, indicating potential sites for nephrotoxic injury.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First report of the use of mucosal swabs of the palatine tonsillar crypt for detection of <i>Mycoplasma bovis</i> in naturally infected calves.

New Zealand veterinary journal·2020

Same author

L-type amino-acid transporter 1 (LAT1): a therapeutic target supporting growth and survival of T-cell lymphoblastic lymphoma/T-cell acute lymphoblastic leukemia.

Leukemia·2014

Same author

Google unveils a glimpse of allergic rhinitis in the real world.

Allergy·2014

Same author

Fractalkine and its receptor mediate extracellular matrix accumulation in diabetic nephropathy in mice.

Diabetologia·2013

Same author

α-secretase cleaved amyloid precursor protein (APP) accumulates in cholinergic dystrophic neurites in normal, aged hippocampus.

Neuropathology and applied neurobiology·2013

Same author

High glucose increases mesangial lipid accumulation via impaired cholesterol transporters.

Transplantation proceedings·2012

Area of Science:

Nephrology
Biochemistry
Toxicology

Background:

Elevated lipid peroxides (LPO) indicate increased reactive oxygen metabolites, key pathological mediators in kidney disease.
Quantifying LPO is crucial for understanding renal pathophysiology and site-specific insults.

Purpose of the Study:

To develop a sensitive microassay for measuring LPO in kidney tissue.
To investigate basal and stimulated LPO levels along the rat nephron.
To explore the role of protein kinase C (PKC) in LPO formation.

Main Methods:

Developed a fluorometric microassay to quantify malondialdehyde-thiobarbituric acid adducts in <1 microgram of tissue protein.
Measured basal LPO levels in different segments of the rat nephron.
Stimulated LPO levels using phorbol 12-myristate 13-acetate (PMA) and assessed inhibition with sphingosine (PKC inhibitor).

Main Results:

Proximal tubules exhibited higher basal LPO levels (approx. 0.2 pmol/mm) than distal segments (approx. 0.02 pmol/mm), with S3 showing the highest concentration (2.2 +/- 0.1 pmol/microgram protein).
PMA significantly stimulated LPO in glomeruli, S3, and S2 segments.
Sphingosine completely blocked PMA-induced LPO increase, implicating PKC in LPO generation.

Conclusions:

The developed LPO assay is applicable to nephron segments for evaluating site-specific nephrotoxic insults.
PKC activation plays a role in mediating LPO formation within the kidney.
This assay aids in understanding the mechanisms of renal pathophysiology and nephrotoxicity.

Related Experiment Videos