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In vitro assays for kinetoplastid U insertion-deletion editing and associated activities
Kenneth Stuart1, Reza Salavati, Robert P Igo
1Seattle Biomedical Research Institute, and Department of Pathobiology, University of Washington, Seattle, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 23, 2004
Abstract:
This chapter describes biochemical assays that have been used in analyzing RNA editing in kinetoplastid mitochondria and to characterize the general mechanism of editing by the editosome. Studies using these assays have shown that the characteristics of each activity contribute to editing site selection, U addition and removal, and RNA ligation resulting in accurately edited mRNAs.

