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Updated: Aug 24, 2026
Essential Components of Borreliella (Borrelia) burgdorferi In Vitro Transcription Assays
Published on: July 22, 2022
Crystallization and preliminary crystallographic analysis of BbCRASP-1, a complement regulator-acquiring surface
Frank S Cordes1, Peter Kraiczy, Pietro Roversi
1Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, England.
Abstract:
Borrelia burgdorferi is the causative agent of Lyme disease. Serum-resistant strains of the pathogen are able to reduce the host's immune response to infection by recruiting fluid-phase complement regulators from the serum. B. burgdorferi complement regulator-acquiring surface protein-1 (BbCRASP-1) binds factor H and factor-H-like protein-1 to the bacterial surface, where they actively down-regulate complement response. Crystals of native and selenomethionine-substituted BbCRASP-1 have been obtained and a native data set to 2.7 A as well as selenomethionine MAD data to 3.2 A resolution have been collected. The selenium substructure has been solved and initial phases have been refined to 3.0 A by density-modification methods. Model building and refinement are under way.
