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Related Experiment Videos

Vancomycin resistance in Enterococcus gallinarum.

S Vincent1, P Minkler, B Bincziewski

  • 1Research Service, Department of Veterans Affairs Medical Center, Case Western Reserve University, Cleveland, Ohio 44106.

Antimicrobial Agents and Chemotherapy
|July 1, 1992
PubMed
Summary
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Enterococcus gallinarum exhibits vancomycin resistance, but remains susceptible to other glycopeptides. Beta-lactam antibiotics, particularly those targeting penicillin-binding protein 6, can inhibit this resistance, suggesting PBP 6

Area of Science:

  • Microbiology
  • Antibiotic Resistance
  • Biochemistry

Background:

  • Enterococcus gallinarum strains display constitutive vancomycin resistance.
  • This resistance is characterized by moderate vancomycin Minimum Inhibitory Concentration (MIC) of 16 µg/ml.
  • E. gallinarum remains susceptible to other glycopeptides like teicoplanin and actaplanins.

Purpose of the Study:

  • To investigate the mechanism of vancomycin resistance in Enterococcus gallinarum.
  • To explore the role of penicillin-binding protein 6 (PBP 6) in this resistance.
  • To identify potential enzymatic activity in E. gallinarum membranes related to resistance.

Main Methods:

  • Growth and peptidoglycan synthesis inhibition assays were used to assess resistance.
  • Penicillin-binding protein 6 (PBP 6) competition experiments evaluated drug interactions.

Related Experiment Videos

  • Bioassay and high-performance liquid chromatography (HPLC) analyzed membrane enzymatic activity.

    • Main Results:

    • Vancomycin resistance in E. gallinarum was inhibited by beta-lactam antibiotics targeting PBP 6.
    • Spontaneous mutants showed increased resistance to beta-lactam inhibition of vancomycin resistance.
    • E. gallinarum membranes possess D,D-carboxypeptidase-like activity, inhibited by beta-lactams and PBP 6 saturation.

    Conclusions:

    • Penicillin-binding protein 6 (PBP 6) is likely involved in vancomycin resistance in E. gallinarum.
    • The observed D,D-carboxypeptidase activity in E. gallinarum membranes may be attributed to PBP 6.
    • Beta-lactam antibiotics show potential for modulating vancomycin resistance in E. gallinarum.