Related Experiment Video
Updated: Aug 24, 2026
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)
Published on: January 2, 2014
Minimum inhibitory concentration (MIC) versus minimum biofilm eliminating concentration (MBEC) in evaluation of
Farshad Sepandj1, Howard Ceri, Allan Gibb
1Department of Medicine, University of Calgary, Calgary, Alberta, Canada. fsepandj@shaw.ca
Objective:
Minimum inhibitory concentration (MIC) and minimum biofilm eliminating concentration (MBEC) results were compared to determine changes in the pattern of antibiotic sensitivity of gram-negative bacilli from the planktonic to the biofilm phase of growth.
Methodology:
The MIC and MBEC assays were conducted on stored isolates obtained from patients presenting with peritoneal dialysis-related gram-negative peritonitis with Escherichia coli or Pseudomonas.
Results:
The antibiotic sensitivities of planktonic organisms tested by the MIC assays were significantly higher than the antibiotic sensitivities of the same organisms in their biofilm state, as tested by the MBEC assays.
Conclusions:
In their biofilm state, gram-negative bacteria are much less susceptible to antibiotics compared to their antibiotic susceptibility in the planktonic state.
More Related Videos
09:26Antibiotic Efficacy Testing in an Ex vivo Model of Pseudomonas aeruginosa and Staphylococcus aureus Biofilms in the Cystic Fibrosis Lung
Published on: January 22, 2021
07:46Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
Published on: June 5, 2012
Related Concept Videos
01:28Antimicrobial Effectiveness
01:25Clinical Significance of Antibiotic Resistance