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Related Experiment Videos

Flow cytometry-based methods for apoptosis detection in lymphoid cells.

Owen Williams1

  • 1Molecular Hematology and Cancer Biology Unit, Institute of Child Health, University College London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|April 24, 2004
PubMed
Summary

Apoptosis, programmed cell death, is vital for lymphocyte development and homeostasis. Flow cytometry effectively quantifies cellular changes during lymphocyte apoptosis, aiding research.

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Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Apoptosis, or programmed cell death, is essential for lymphocyte development, selection, and maintaining homeostasis.
  • This active cell death process involves specific biochemical pathways and distinct morphological and functional cellular changes.

Purpose of the Study:

  • To highlight the critical role of apoptosis in lymphocyte biology.
  • To describe the characteristic cellular alterations during lymphocyte apoptosis.
  • To present flow cytometry as a method for quantifying these alterations.

Main Methods:

  • Characterization of biochemical pathways activating apoptosis.
  • Observation of cellular morphological changes (shrinkage, blebbing, condensation).
  • Quantification of DNA fragmentation, mitochondrial membrane potential, and plasma membrane changes.

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Main Results:

  • Apoptosis involves cell shrinkage, membrane blebbing, and nuclear condensation.
  • Biochemical pathways lead to DNA fragmentation and mitochondrial dysfunction.
  • Plasma membrane alterations are also characteristic of lymphocyte apoptosis.

Conclusions:

  • Apoptosis is a fundamental process in lymphocyte regulation.
  • Flow cytometry provides a robust method for assessing multiple parameters of lymphocyte apoptosis simultaneously.
  • Quantitative analysis of these cellular changes is crucial for understanding lymphocyte function and dysfunction.