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Histamine dilutions modulate basophil activation.

P Belon1, J Cumps, M Ennis

  • 1Boiron, 20 rue de la Libération, 69110 Sainte-Foy-Les-Lyon, France.

Inflammation Research : Official Journal of the European Histamine Research Society ... [Et Al.]
|April 24, 2004
PubMed
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High dilutions of histamine were found to inhibit human basophil activation, a finding reproducible across multiple labs and methods. This effect, specific to histamine and its H2 receptor interaction, warrants further investigation.

Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • Investigating the reproducible inhibition of basophil activation by high dilutions of histamine.
  • Exploring the potential of specific molecular interactions in immune response modulation.

Purpose of the Study:

  • To investigate the action of high dilutions of histamine on human basophil activation.
  • To confirm findings across multiple research centers and methodologies.

Main Methods:

  • Multi-center blinded study assessing basophil activation via alcian blue staining, histamine release, and CD63 expression.
  • Flow cytometry used for confirmation in independent laboratories.
  • Evaluation of H2 receptor antagonists (cimetidine, ranitidine) and histidine.

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Main Results:

  • High dilutions of histamine (10^-30 to 10^-38 M) inhibited basophil activation, particularly at lower stimulation levels.
  • Inhibitory effects were confirmed by flow cytometry and histamine release assays.
  • Cimetidine reversed histamine-induced CD63 expression inhibition, while ranitidine did not; histidine showed no activity.

Conclusions:

  • High dilutions of histamine demonstrate a specific inhibitory effect on basophil activity, confirmed by multiple experimental approaches.
  • The observed inhibition is mediated via H2 receptors and is specific to histamine.
  • The underlying mechanism remains unexplained, necessitating further research.