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Modulation of intracellular Na+ concentration by BmK AS, a scorpion toxin, in B104 cell line
Zhi-Yong Tan1, Jin Chen, Xing-Hua Feng
1Institute of Physiology, Shanghai Institute of Biological Science, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, PR China.
Abstract:
ABSL Effects of BmK AS, a toxin from scorpion Buthus mortensi Karsch and an activator of skeletal muscle RyRs, on the intracellular Na+ concentration have been investigated in the B104 neuroblastoma cell line by fluorescence digital imaging techniques. The intracellular Na+ concentration was elevated by 500 nM BmK AS significantly over a 30 min period, and the effect could be enhanced by addition of ouabain. In addition, BmK AS induced a biphasic modulation of [Na+]i in the dose-dependent range from 20 to 500 nM in the presence of ouabain. The results suggest that BmK AS may modulate intracellular signals by altering the intracellular Na+ concentration in the B104 cells.
