Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Encephalitozoon cuniculi antibodies in a specific-pathogen-free rabbit unit.

J E Bywater, B S Kellett

    Infection and Immunity
    |August 1, 1978
    PubMed
    Summary

    Encephalitozoon cuniculi antibodies were found in a specific-pathogen-free rabbit colony. A new India ink test improved detection, revealing higher infection rates and transmission patterns.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The indirect india-ink immunoreaction for detection of antibodies to Encephalitozoon cuniculi in rat and mouse serum.

    Laboratory animals·1980
    Same author

    Encephalitozoon cuniculi antibodies in commercially-available rabbit antisera and serum reagents.

    Laboratory animals·1980
    Same author

    Humoral immune response to natural infection with Encephalitozoon cuniculi in rabbits.

    Laboratory animals·1979
    Same author

    India-ink immunoreaction for encephalitozoonosis: phototechnique.

    Laboratory animals·1979
    Same author

    Bacterial growth in ampholytic disinfectant solutions.

    Laboratory animals·1979
    Same author

    Is encephalitozoonosis a zoonosis?

    Laboratory animals·1979

    Area of Science:

    • Veterinary Immunology
    • Parasitology
    • Animal Health

    Background:

    • Encephalitozoon cuniculi (E. cuniculi) is a microsporidian parasite affecting rabbits.
    • Previous serological methods detected low prevalence (approx. 5%) in SPF rabbit colonies.
    • Limitations in small sample sizes hindered accurate prevalence assessment.

    Purpose of the Study:

    • To discover and characterize E. cuniculi infection in a specific-pathogen-free (SPF) rabbit colony.
    • To implement a more sensitive diagnostic method for accurate prevalence determination.
    • To investigate age and sex-related susceptibilities and transmission dynamics.

    Main Methods:

    • Development and application of an India ink immunoreaction test for E. cuniculi antibody detection.
    • 100% screening of a colony (>700 rabbits) with repeat testing after 4 weeks.
    • Culling of positive reactors to control disease spread.
    • Collating new test results with historical sample data.

    Main Results:

    • The India ink immunoreaction test enabled visualization of both positive and negative reactions.
    • A significantly higher prevalence of E. cuniculi infection was identified compared to previous estimates.
    • Data allowed for the observation of age and sex susceptibilities.
    • Insights into the transmission mode of naturally occurring E. cuniculi disease were gained.

    Conclusions:

    • The India ink immunoreaction test is a valuable tool for E. cuniculi antibody detection in rabbits.
    • SPF rabbit colonies can harbor E. cuniculi, necessitating robust screening protocols.
    • Understanding transmission and susceptibility aids in disease management and prevention strategies.

    Related Experiment Videos