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Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
Published on: September 1, 2013
4Pi-microscopy of the Golgi apparatus in live mammalian cells
Alexander Egner1, Sophie Verrier, Alexander Goroshkov
1Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, High Resolution Optical Microscopy Group, 37077 Goettingen, Germany.
Journal of Structural Biology
|April 28, 2004
Abstract:
We report the applicability of 4Pi-microscopy to live mammalian cells. Controlled interference of the counterpropagating wavefronts is possible despite the slight variations in cellular refractive index. Superresolved 3D-fluorescence imaging is exemplified with the first representation of the Golgi apparatus in a live cell at approximately 100 nm resolution.

