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The pentose phosphate pathway in Trypanosoma cruzi
Dante A Maugeri1, Juan J Cazzulo
1Instituto de Investigaciones Biotecnologicas/INTECH, Universidad Nacional de General San Martin/CONICET, Av. Gral Paz s/n, INTI, edificio 24, Av. General Paz y Albarellos, Casilla de Correo 30, 1650 San Martin, Buenos Aires, Argentina.
Abstract:
The pentose phosphate pathway has been studied in Trypanosoma cruzi, Clone CL Brener. Functioning of the pathway was demonstrated in epimastigotes by measuring the evolution of (14)CO(2) from [1-(14)C] or [6-(14)C]D-glucose. Glucose consumption through the PPP increased from 9.9% to 20.4% in the presence of methylene blue, which mimics oxidative stress. All the enzymes of the PPP are present in the four major developmental stages of the parasite. Subcellular localisation experiments suggested that the PPP enzymes have a cytosolic component, predominant in most cases, although all of them also seem to have organellar localisation(s).