Processing of plasmid DNA with ColE1-like replication origin
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Updated: Aug 24, 2026

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks
Published on: November 25, 2015
Zhijun Wang1, Zhenghong Yuan, Ulrich R Hengge
1Key Laboratory of Medical Molecular Virology, Shanghai Medical College, Fudan University, 200032 Shanghai, People's Republic of China. zjwang@shmu.edu.cn
Abstract:
With the increasing utilization of plasmid DNA as a biopharmaceutical drug, there is a rapidly growing need for high quality plasmid DNA for drug applications. Although there are several different kinds of replication origins, ColE1-like replication origin is the most extensively used origin in biotechnology. This review addresses problems in upstream and downstream processing of plasmid DNA with ColE1-like origin as drug applications. In upstream processing of plasmid DNA, regulation of replication of ColE1-like origin was discussed. In downstream processing of plasmid DNA, we analyzed simple, robust, and scalable methods, which can be used in the efficient production of pharmaceutical-grade plasmid DNA.
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