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Updated: Aug 24, 2026

Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+
Published on: May 19, 2017
Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane
G Bartolommei1, F Tadini Buoninsegni, M R Moncelli
1Laboratory of Bioelectrochemistry, Chemistry Department, University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino, Florence, Italy.
Abstract:
Sarcoplasmic reticulum (SR) native vesicles incorporating Ca-ATPase are adsorbed on a solid-supported lipid membrane (SSM). Upon adsorption, the ion pumps are chemically activated by concentration jumps of ATP and the capacitive current transients generated by SR Ca-ATPase are measured under potentiostatic conditions. The Michaelis-Menten constant, K(M), for ATP is evaluated by varying the concentration of ATP in the activating solution. This preliminary result shows that ion transport by SR Ca-ATPase can be suitably investigated by a technique based on concentration jumps on an SSM.
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