Related Experiment Video
Updated: Aug 24, 2026
12:14
A Converging Strategy for the Generation of a Virtually Sequenced cDNA Library from Unreferenced Pacific Oysters
Published on: June 13, 2019
Molecular cloning and sequencing of shrimp (Penaeus monodon) penaeidin-5 cDNA
Jyh-Yih Chen1, Chia-Yu Pan, Ching-Ming Kuo
1Marine Research Station, Institute of Zoology, Academia Sinica, 23-10, Dah-Uen Road, Jiaushi, Ilan 262, Taiwan, ROC.
Fish & Shellfish Immunology
|April 28, 2004
Abstract
No abstract available in PubMed .
Related Concept Videos
02:35RACE - Rapid Amplification of cDNA Ends
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
Since the...
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
01:44Complementary DNA
Overview