Determination of lipid hydroperoxides in serum iodometry and high performance liquid chromatography compared

It is postulated that lipid peroxidation plays a role in the pathogenesis of a variety of diseases. Efforts have therefore been made to develop reliable and practicable procedures for quantifying lipid peroxidation products such as lipid hydroperoxides in biological specimens. An iodometric cholesterol colour reagent (Merck, Darmstadt, Germany) can be used to measure lipid hydroperoxides in isolated low density lipoproteins without lipid extraction. This method has been validated with respect to its analytical performance and suitability for serum samples by comparing it with a high performance liquid chromatography technique. The method was found to have acceptable performance characteristics with aqueous fatty acid hydroperoxide solutions (linoleic acid) and isolated low density lipoproteins, but it cannot be applied to native serum samples without extraction of lipids.