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Related Experiment Videos

It's green outside: tracking cell surface proteins with pH-sensitive GFP.

Michael C Ashby1, Kyoko Ibaraki, Jeremy M Henley

  • 1MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.

Trends in Neurosciences
|April 28, 2004
PubMed
Summary

pH-dependent green fluorescent protein (GFP) variants, like pHluorins, offer non-invasive monitoring of cellular processes. These advanced GFP tools enable high-resolution study of neuronal activity, including exocytosis and endocytosis.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Green fluorescent protein (GFP) and its variants are essential tools in biological research.
  • Understanding cellular dynamics requires advanced imaging techniques.

Purpose of the Study:

  • To review the development and applications of pH-dependent GFP variants.
  • To highlight the potential of pHluorins for studying neuronal processes.

Main Methods:

  • Review of literature on GFP variants and their applications.
  • Focus on pH-dependent variants like pHluorins.
  • Analysis of their use in monitoring cellular events.

Main Results:

  • pH-dependent GFP variants offer significant advantages for biological research.

Related Experiment Videos

  • pHluorins enable non-invasive, high-resolution monitoring of exocytosis, endocytosis, and protein surface expression in neurons.

    • Conclusions:

    • pH-dependent GFP variants, particularly pHluorins, represent a promising advancement in biological imaging.
    • These tools facilitate detailed investigation of neuronal function with unprecedented spatial and temporal accuracy.