It's green outside: tracking cell surface proteins with pH-sensitive GFP
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Updated: Aug 24, 2026

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
Published on: February 5, 2016
Michael C Ashby1, Kyoko Ibaraki, Jeremy M Henley
1MRC Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.
Abstract:
Green fluorescent protein (GFP) and mutated GFP variants have proved to be immensely powerful tools that have had a profound impact on research in biological sciences. This review considers the development, use and future implications of pH-dependent GFP variants (e.g. pHluorins). These proteins hold considerable promise for the relatively non-invasive monitoring of events such as exocytosis, endocytosis and protein surface expression in living neurons with high spatial and temporal resolution.
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