Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pulling-speed-dependent force-extension profiles for semiflexible chains.

Nam-Kyung Lee1, D Thirumalai

  • 1Department of Physics and Institute of Fundamental Physics, Sejong University, Seoul, South Korea. lee@sejong.ac.kr

Biophysical Journal
|April 28, 2004
PubMed
Summary

We explore nonequilibrium stretching of DNA using theory and simulations. Increased pulling speed alters chain force, revealing a stem-flower mechanism at high speeds, observable in DNA at ~100 microm/s.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A novel Tie2xVEGF bispecific antibody fusion demonstrates enhanced therapeutic efficacy through direct Tie2 activation and VEGF neutralization.

Journal of translational medicine·2026
Same author

Connecting multiway enhancer-promoter interactions to changes in gene expression in cancer.

bioRxiv : the preprint server for biology·2026
Same author

Green-Synthesized rGO-CeO<sub>2</sub> Nanocomposites for Enhanced Visible-Light Photodegradation of Eosin Y.

Chemistry, an Asian journal·2026
Same author

Aggregation of polyampholytes: Influence of salt concentration.

The Journal of chemical physics·2026
Same author

Droplet growth, Ostwald's rule, and emergence of order in Fused in Sarcoma.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Hepatocyte-Targeted Epicatechin Nanoparticles Promote Autophagy and Enhance Mitochondrial Function in Metabolic Dysfunction-Associated Steatotic Liver Disease.

International journal of nanomedicine·2025

Area of Science:

  • Biophysics
  • Polymer Physics
  • Molecular Dynamics

Background:

  • Semiflexible chains, such as DNA, are modeled as worm-like chains.
  • Understanding their mechanical properties under stretching is crucial for molecular biology.
  • Nonequilibrium dynamics can significantly alter chain behavior compared to equilibrium conditions.

Purpose of the Study:

  • To theoretically and computationally investigate the nonequilibrium stretching of semiflexible chains.
  • To determine the force-extension curves as a function of pulling speed.
  • To elucidate the underlying mechanisms governing chain dynamics under rapid stretching.

Main Methods:

  • A self-consistent dynamical variational approach was used for theoretical calculations.
  • Langevin simulations of extensible worm-like chain models were performed.

Related Experiment Videos

  • Force-extension curves were analyzed as a function of pulling speed (v(0)).

    • Main Results:

    • Theoretical predictions for force-extension curves show good agreement with simulation results.
    • Stretching force increases with pulling speed and exhibits nonmonotonic behavior with increasing persistence length.
    • At high pulling speeds, tension propagation follows the Brochard-Wyart stem-flower mechanism.

    Conclusions:

    • Nonequilibrium effects significantly influence the stretching behavior of semiflexible chains.
    • The stem-flower mechanism governs tension propagation at high pulling speeds.
    • Observable nonequilibrium effects in double-stranded DNA require high pulling speeds (~100 microm/s).