Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Size effects on diffusion processes within agarose gels.

Nicolas Fatin-Rouge1, Konstantin Starchev, Jacques Buffle

  • 1Analytical and Biophysical Environmental Chemistry, University of Geneva, Geneva, Switzerland. nicolas.fatin-rouge@wanadoo.fr

Biophysical Journal
|April 28, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative study of zero-valent mercury immobilization by solidification/stabilization in a limestone backfill model.

Chemosphere·2026
Same author

Complexation in Aqueous Solution of a Hydrophobic Polyanion (PSSNa) Bearing Different Charge Densities with a Hydrophilic Polycation (PDADMAC).

Polymers·2022
Same author

Assessment of shear-thinning fluids and strategies for enhanced in situ removal of heavy chlorinated compounds-DNAPLs in an anisotropic aquifer.

Journal of hazardous materials·2022
Same author

Controlled treatment of a high velocity anisotropic aquifer model contaminated by hexachlorocyclohexanes.

Environmental pollution (Barking, Essex : 1987)·2020
Same author

Assessing natural clays of a contaminated site to stabilize and reduce the ecotoxicity of a coal tar.

Ecotoxicology and environmental safety·2019
Same author

Comparative assessment of a foam-based oxidative treatment of hydrocarbon-contaminated unsaturated and anisotropic soils.

Chemosphere·2019

Diffusion of nanoparticles in agarose gel exhibits anomalous behavior, particularly when particles larger than 0.4 times the pore size become trapped. This study used fluorescence correlation spectroscopy to analyze particle movement in gel pores.

Area of Science:

  • Biophysics
  • Materials Science
  • Physical Chemistry

Background:

  • Agarose gels serve as valuable models for biological environments like cytoplasm and biofilms.
  • Understanding diffusion within these gels is crucial for various biological and material science applications.
  • Fluorescence correlation spectroscopy (FCS) offers high sensitivity and resolution for probing nanoscale diffusion.

Purpose of the Study:

  • To investigate nanoparticle diffusion dynamics in agarose gels.
  • To determine the relationship between particle size and diffusion behavior within the gel matrix.
  • To compare experimental findings with theoretical models and simulations.

Main Methods:

  • Utilized fluorescence correlation spectroscopy (FCS) to track nanoparticles ranging from 1 to 140 nm.

Related Experiment Videos

  • Employed varying concentrations of agarose gel to model different pore sizes.
  • Analyzed diffusion anomalies and calculated fractal dimensions of particle movement.

    • Main Results:

    • Determined the maximum hydrodynamic radius of mobile particles in 1.5% agarose gel to be 70 nm.
    • Observed anomalous diffusion behavior, characterized by a diverging fractal dimension for larger entrapped particles (reduced size > 0.4).
    • Found agreement between experimental fractal exponents and 3D Monte Carlo simulations, with a systematic offset attributed to particle-fiber interactions.

    Conclusions:

    • Nanoparticle diffusion in agarose gels is anomalous and size-dependent.
    • Particle entrapment and interactions with the gel matrix significantly influence diffusion.
    • Experimental results highlight the need to account for non-elastic interactions in diffusion models for porous media.