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Updated: Aug 9, 2026

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Atomic mean-square displacements in proteins by molecular dynamics: a case for analysis of variance
Luca Maragliano1, Grazia Cottone, Lorenzo Cordone
1National Institute for the Physics of Matter and Physics Department, University of Rome, La Sapienza, 00185 Rome, Italy. maragliano.luca@unimo.it
Abstract:
Information on protein internal motions is usually obtained through the analysis of atomic mean-square displacements, which are a measure of variability of the atomic positions distribution functions. We report a statistical approach to analyze molecular dynamics data on these displacements that is based on probability distribution functions. Using a technique inspired by the analysis of variance, we compute unbiased, reliable mean-square displacements of the atoms and analyze them statistically. We applied this procedure to characterize protein thermostability by comparing the results for a thermophilic enzyme and a mesophilic homolog. In agreement with previous experimental observations, our analysis suggests that the proteins surface regions can play a role in the different thermal behavior.
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