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Related Experiment Videos

Root-end filling materials alter fibroblast differentiation.

S Bonson1, B G Jeansonne, T E Lallier

  • 1Department of Endodontics, Center of Excellence in Oral and Craniofacial Biology, Louisiana State University Health Science Center, School of Dentistry, New Orleans, LA 70119, USA.

Journal of Dental Research
|April 28, 2004
PubMed
Summary

Root-end filling materials like mineral trioxide aggregate (MTA) and hybrid ionomer composite resin (HICR) impact fibroblast survival and differentiation. MTA generally supports fibroblast proliferation and differentiation, while HICR shows inhibitory effects.

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Area of Science:

  • Biomaterials Science
  • Endodontics
  • Cell Biology

Background:

  • Root-end filling materials are crucial in endodontic surgery but their soft tissue effects are unclear.
  • Investigating the differential biological responses of periodontal ligament (PDL) and gingival fibroblasts to these materials is essential.

Purpose of the Study:

  • To evaluate the effects of common root-end filling materials on fibroblast survival, proliferation, and differentiation.
  • To compare the biocompatibility and cellular responses induced by materials like mineral trioxide aggregate (MTA) and hybrid ionomer composite resin (HICR).

Main Methods:

  • Co-culture experiments assessed initial cytotoxicity of root-end filling materials on PDL and gingival fibroblasts.
  • Cellular responses, including proliferation, alkaline phosphatase activity, and gene expression (pleiotrophin, periostin), were analyzed.

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  • Material pre-conditioning (washing) was performed to evaluate reduced cytotoxicity.

    • Main Results:

    • Initially cytotoxic materials showed reduced toxicity after pre-conditioning with MTA or HICR.
    • PDL fibroblasts exhibited enhanced proliferation on MTA and survival on amalgam compared to gingival fibroblasts.
    • MTA promoted alkaline phosphatase activity, whereas HICR inhibited it, indicating differential effects on fibroblast differentiation.

    Conclusions:

    • Root-end filling materials exert distinct effects on periodontal fibroblast differentiation.
    • Material composition significantly influences fibroblast behavior, impacting outcomes in endodontic surgery.
    • MTA and HICR demonstrate varied biocompatibility profiles, affecting key cellular processes in adjacent tissues.