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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
Effect of 2'-deoxyisoguanosine on the growth of human tumor and normal cell lines
Radoslawa Nowak1, Maria Sawardo-Rochowska, Zygmunt Kazimierczuk
1Department of Molecular Biology, Maria Sklodowska-Curie Memorial Cancer Center, Institute of Oncology, Warsaw, Poland. radka@coi.waw.pl
Aim:
To test the effect of 2'-deoxyisoguanosine as a specific substrate of HIV reverse transcriptase on the growth of cultured normal and cancer cells.
Methods:
The effect of 2'-deoxyisoguanosine on the growth of cells of two normal (telomerase-negative) and five cancer cell lines have been tested. Cell viability was assessed by MTT assay.
Results:
We have found that this nucleoside analogue has low potency and specificity in inhibiting tumor cell growth. IC50 ranged from 0.5 mM to 2 mM for tumor cells, and from 1 mM to more than 4 mM for normal cells.
Conclusions:
2'-deoxyisoguanosine has low potency and specificity in inhibiting tumor cell growth, similar to other telomerase inhibitors.
