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Expression of multi-drug resistance genes (mdr1, mrp1, bcrp) in primary oral squamous cell carcinoma

R E Friedrich1, C Punke, A Reymann

  • 1Klinik für Mund-, Kiefer- und Gesichtschirurgie, Universitaetsklinikum Hamburg-Eppendorf, Universitaet Hamburg, Germany. rfriedri@uke.uni-hamburg.de

In Vivo (Athens, Greece)
|April 29, 2004
PubMed
Abstract

Insights

Resistance genes like mdr1, mrp1, and bcrp can be upregulated in oral squamous cell carcinoma (OSCC), impacting chemotherapy effectiveness. BCRP expression correlates with advanced stages and poorer survival in OSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Chemotherapeutic resistance in cancer is often mediated by the expression of specific genes.
  • Oral squamous cell carcinoma (OSCC) poses a significant challenge in cancer treatment due to potential treatment ineffectiveness.

Purpose of the Study:

  • To investigate the expression of known drug resistance genes in OSCC.
  • To correlate the expression of these genes with clinico-pathological findings and patient outcomes.

Main Methods:

  • Expression analysis of mdr1, mrp1, and bcrp genes using reverse transcriptase PCR in 45 primary OSCC samples.
  • Immunohistochemical validation of gene products.
  • Correlation with TNM stage, HPV infection, and p53 mutations.

Main Results:

  • Gene expression was independent of age, sex, tumor site, HPV, and p53 mutations.
  • BCRP expression showed a 2.7-fold increase in Stage IV OSCC compared to Stage III.
  • Increased expression of mdr1, mrp1, and BCRP was associated with loss of differentiation and shorter survival periods.

Conclusions:

  • Multi-drug resistance genes are frequently upregulated in OSCC, particularly in advanced stages (Stage IV).
  • Determining the expression of these resistance genes may aid in developing targeted therapies and improving treatment strategies for OSCC.

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