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Related Experiment Videos

Multivitamin production in Lactococcus lactis using metabolic engineering.

Wilbert Sybesma1, Catherine Burgess, Marjo Starrenburg

  • 1Department of Flavor, Nutrition and Natural Ingredients, Wageningen Centre for Food Sciences, NIZO food research, Kernhemseweg 2, P.O. Box 20, 6710 BA Ede, The Netherlands.

Metabolic Engineering
|April 29, 2004
PubMed
Summary

Lactococcus lactis was engineered to overproduce folate and riboflavin, essential B vitamins. These enhanced dairy starters can fortify foods, addressing common B-vitamin deficiencies, particularly for individuals with MTHFR gene mutations.

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Area of Science:

  • Microbiology and Biotechnology
  • Nutritional Science
  • Metabolic Engineering

Background:

  • Lactococcus lactis, a dairy starter bacterium, possesses the capability to synthesize folate (vitamin B11) and riboflavin (vitamin B2).
  • B-vitamin deficiencies are prevalent globally, even in developed nations, and specific genetic variations like MTHFR mutations can exacerbate these deficiencies.

Purpose of the Study:

  • To genetically modify Lactococcus lactis for simultaneous overproduction of both folate and riboflavin.
  • To develop enhanced starter cultures for food fortification to address widespread B-vitamin deficiencies.

Main Methods:

  • Directed mutagenesis and selection were employed, focusing on the riboflavin biosynthetic pathway using the analogue roseoflavin.
  • Metabolic engineering strategies, including constitutive overproduction of GTP cyclohydrolase I, were applied to enhance folate synthesis.

Related Experiment Videos

  • Characterization of a spontaneous L. lactis mutant (NZ9000) exhibiting enhanced riboflavin production due to a regulatory gene mutation.

    • Main Results:

    • A spontaneous mutant of L. lactis was isolated, transitioning from a riboflavin consumer to a producer via a single base change in regulatory regions.
    • Constitutive overproduction of GTP cyclohydrolase I in the riboflavin-producing strain led to increased folate production.
    • Simultaneous overproduction of both folate and riboflavin was achieved through metabolic engineering of L. lactis.

    Conclusions:

    • Engineered L. lactis strains can simultaneously overproduce folate and riboflavin, offering a novel approach to food fortification.
    • Fermented foods utilizing these multivitamin-producing starters can help compensate for common B-vitamin deficiencies.
    • These fortified foods hold particular promise for individuals with MTHFR gene mutations, a common genetic factor affecting folate metabolism.