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Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
Rapid preparation of vaccinia virus DNA template for analysis and cloning by PCR
1Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 29, 2004
Abstract:
This chapter describes the preparation of template DNA from poxvirus-infected cells, plaques, or crude virus stocks for polymerase chain reaction (PCR) amplification. The advantages of this technique are that it is rapid, inexpensive, and, most importantly, reliable, requiring only centrifugation, detergent, and protease treatment. The template preparation is suitable for PCR amplification for screening viruses, cloning, transfection, and DNA sequencing.

