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Vaccinia Reporter Viruses for Quantifying Viral Function at All Stages of Gene Expression
Published on: May 15, 2014
An in vitro transcription system for studying vaccinia virus late genes
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 29, 2004
Abstract:
This chapter describes a protocol that allows accurate in vitro transcription of vaccinia virus late genes. In this method, extracts are made from vaccinia virus-infected cells and used as enzyme sources to produce mRNAs from plasmid templates containing late gene promoter sequences.
