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Updated: Aug 24, 2026
Measurement of Chitinase Activity in Biological Samples
Published on: August 22, 2019
Chitosanase activity in Bacillus thuringiensis
R Cruz Camarillo1, O Sánchez Pérez, N G Rojas Avelizapa
1Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politecnico National, México 11340, DF, México. cruzcamarillo@hotmail.com
Abstract:
The ability to produce extracellular chitosanase (EC 3.2.1.132) was found by plate assays in 18 (23%) out of 77 crystalliferous strains of Bacillus thuringiensis. The best chitosanase producer was selected after the growth chosen in a liquid medium with colloidal chitosan as carbon source. Enzyme production was optimized (a 4-d incubation at 32 degrees C with shaking in a medium of pH 6.5 with 4% colloidal chitosan) and the enzyme was partially characterized. This is the first report on the chitosanase of B. thuringiensis.
