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Chitosanase activity in Bacillus thuringiensis.

R Cruz Camarillo1, O Sánchez Pérez, N G Rojas Avelizapa

  • 1Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politecnico National, México 11340, DF, México. cruzcamarillo@hotmail.com

Folia Microbiologica
|April 30, 2004
PubMed
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Researchers discovered extracellular chitosanase production in Bacillus thuringiensis strains. The study optimized enzyme production and characterized the enzyme, marking the first report for this bacterium.

Area of Science:

  • Microbiology
  • Enzymology

Background:

  • Chitosanase enzymes are crucial for degrading chitin and chitosan.
  • Bacillus thuringiensis is known for its insecticidal properties but less studied for enzyme production.

Purpose of the Study:

  • To identify and characterize extracellular chitosanase production in Bacillus thuringiensis.
  • To optimize the conditions for enzyme production.

Main Methods:

  • Screening of 77 Bacillus thuringiensis strains using plate assays.
  • Selection of the best producer in a liquid medium with colloidal chitosan.
  • Optimization of incubation time, temperature, pH, and substrate concentration.
  • Partial characterization of the produced enzyme.

Main Results:

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  • Extracellular chitosanase activity was detected in 18 (23%) of the tested strains.
  • Optimal production conditions were determined: 4-day incubation at 32°C, pH 6.5, with 4% colloidal chitosan and shaking.
  • The enzyme was partially characterized.

Conclusions:

  • Bacillus thuringiensis is a potential source of extracellular chitosanase.
  • This study provides the first report and characterization of chitosanase from Bacillus thuringiensis.
  • Further research can explore its biotechnological applications.