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Automated lanes detection and comparison of bacterial electrophoretic protein fingerprints using fast Fourier
1Department of Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Computers and Biomedical Research, an International Journal
|August 1, 1992
Abstract:
A method of computer-automated analysis of bacterial fingerprints produced by electrophoresis of proteins in a one-dimensional slab gel system is described. Proteins were visualized by silver staining. Western blotting, or autoradiography. Gels were recorded with a CCD camera, and after initial manual removal of the unwanted image margins, track margins were identified and extracted and a normalized trace was produced automatically using Fourier routines to smooth plots required for this process. Normalized traces were then compared by Fourier correlation after application of a high-pass step filter.