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Isolation and molecular characterization of a novel type 3 reovirus from a child with meningitis

Kenneth L Tyler1, Erik S Barton, Maria L Ibach

  • 1Department of Neurology, University of Colorado Health Science Center, Denver, Colorado 80262, USA. ken.tyler@uchsc.edu.

The Journal of Infectious Diseases
|April 30, 2004
PubMed

Insights

A novel reovirus strain, T3C/96, was isolated from a child with meningitis. This serotype 3 reovirus can cause encephalitis and suggests a link between reovirus serotype 3 and human meningitis.

Area of Science:

  • Virology
  • Neuroscience

Background:

  • Mammalian reoviruses are non-enveloped viruses with double-stranded RNA genomes.
  • Human reovirus infections are typically asymptomatic.

Observation:

  • A novel reovirus strain, T3/Human/Colorado/1996 (T3C/96), was isolated from a 6.5-week-old child diagnosed with meningitis.
  • Hemagglutination and neutralization assays identified the isolate as a serotype 3 strain.

Findings:

  • Sequence analysis of the S1 gene segment confirmed T3C/96 as a novel reovirus isolate.
  • T3C/96 demonstrated systemic spread and lethal encephalitis in newborn mice after peroral inoculation.

Implications:

  • This study suggests that serotype 3 reoviruses can cause meningitis in humans.
  • The findings highlight the potential neurotropic and pathogenic capabilities of specific reovirus serotypes.

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