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Related Experiment Videos

An algorithm to predict pregnancy in assisted reproduction.

S E M Lewis1, M O'Connell, M Stevenson

  • 1School of Medicine, Obstetrics & Gynaecology, Queen's University, Belfast BT12 6BJ, UK. s.e.lewis@qub.ac.uk

Human Reproduction (Oxford, England)
|May 1, 2004
PubMed
Summary
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Sperm mitochondrial (mt) DNA deletions and nuclear (n) DNA fragmentation are linked to ICSI pregnancy success. Lower levels of these sperm DNA issues correlate with higher pregnancy rates in assisted reproduction.

Area of Science:

  • Reproductive Biology
  • Genetics
  • Assisted Reproductive Technology

Background:

  • Conventional parameters inadequately assess male fertility for Intracytoplasmic Sperm Injection (ICSI).
  • Sperm mitochondrial DNA (mtDNA) deletions and nuclear DNA (nDNA) fragmentation are potential indicators of male infertility.

Purpose of the Study:

  • To investigate the association between sperm mtDNA deletions, nDNA fragmentation, and ICSI outcomes.
  • To determine if these DNA integrity markers predict fertilization and pregnancy rates in ICSI procedures.

Main Methods:

  • Analysis of mtDNA deletions using long PCR in ejaculated and testicular sperm.
  • Assessment of nDNA fragmentation via the alkaline Comet assay.
  • Correlation of DNA integrity markers with fertilization and pregnancy rates in 77 men undergoing ICSI.

Related Experiment Videos

Main Results:

  • Reduced mtDNA deletions and nDNA fragmentation in sperm were associated with successful pregnancies in ICSI.
  • Inverse relationships observed between pregnancy rates and the extent of mtDNA deletions and nDNA fragmentation.
  • No significant correlation found between sperm DNA integrity and fertilization rates.

Conclusions:

  • Sperm mtDNA deletions and nDNA fragmentation are significant predictors of ICSI pregnancy success.
  • These DNA integrity markers are closely associated with achieving pregnancy using both testicular and ejaculated sperm in ICSI.