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Updated: Aug 24, 2026

Mouse Genome Engineering Using Designer Nucleases
12:04

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

p53 designer genes for the modern mouse

Manfred Hergenhahn1, Jun-Li Luo, Monica Hollstein

  • 1Department of Genetic Alterations in Carcinogenesis, German Cancer Research Center, Heidelberg, Germany.

Cell Cycle (Georgetown, Tex.)
|May 1, 2004
PubMed

Insights

Developing humanized p53 mouse models is crucial for cancer treatment research. These models will aid in testing p53-targeting drugs and understanding tumor mutation origins.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The p53 pathway is a critical target for cancer therapy.
  • Understanding p53 mutations is key to developing effective cancer treatments.

Purpose of the Study:

  • To introduce humanized p53 sequences into mouse models.
  • To create informative models for testing p53 rescue drugs.
  • To experimentally explore the causes of human tumor mutations.

Main Methods:

  • Development of genetically engineered mouse strains.
  • Incorporation of humanized p53 sequences with a focus on the DNA-binding domain.

Main Results:

  • The developed mouse models accurately represent the human p53 DNA-binding domain.
  • These models serve as valuable tools for preclinical drug testing.

Conclusions:

  • Humanized p53 mouse models are essential for advancing cancer therapy research.
  • These models facilitate the study of p53's role in tumorigenesis and drug response.

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