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Updated: Aug 24, 2026
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
Published on: May 18, 2018
Plasma intestinal fatty acid binding protein (I-FABP) concentrations increase following intestinal ischemia in pigs
T A Niewold1, M Meinen, J van der Meulen
1Animal Sciences Group, Division of Animal Resources Development, Research Group Animal Physiology and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. theo.niewold@wur.nl
Abstract:
Intestinal fatty acid binding protein (I-FABP) is an intracellular epithelial protein in the intestinal mucosa of many animals. I-FABP appears in the circulation following epithelial damage, and in humans, is proven to be a parameter for damage to the mucosa. In this paper, an ELISA test designed for human I-FABP analysis was used to assay pig blood samples. The test recognized I-FABP cloned from pig small intestine and expressed in Escherichia coli. Furthermore, in our experimental model of (low flow) intestinal ischemia and reperfusion a significant rise in plasma I-FABP concentrations 15-30 min after clamping of the mesenteric artery was demonstrated. This is the first report that in pigs circulating I-FABP is a useful marker for (mild) intestinal injury, and could possibly be used to monitor (intestinal) health in clinical practice.
