Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Targeting HSV amplicon vectors.

Paola Grandi1, Matthew Spear, Xandra O Breakefield

  • 1Departments of Neurology and Radiology, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Charlestown, MA 02129, USA.

Methods (San Diego, Calif.)
|May 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection and characterization of <i>Clostridium botulinum</i> isolated from powdered infant formula.

Frontiers in microbiology·2026
Same author

Detection of mutant cancer-derived circRNAs in extracellular vesicles by rolling circle amplification.

Molecular therapy. Nucleic acids·2026
Same author

2025 Advancing Cell and Gene Therapies for Cancer post-meeting report.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Enhanced-mRNA Delivery Using Ultrasound-Delivered Anchors for Bioorthogonal Ligation.

Angewandte Chemie (International ed. in English)·2026
Same author

BET Isoform Selectivity through Diverse Linkers for Bivalent Inhibitors: GSK785, a BRD2/4-Selective Bivalent BET Inhibitor.

Journal of medicinal chemistry·2025
Same author

Extracellular vesicles for the delivery of gene therapy.

Nature reviews bioengineering·2025

Researchers modified herpes simplex virus type-1 (HSV-1) amplicon vectors to target specific cells. By altering glycoprotein C, they enhanced gene delivery efficiency to targeted cells without compromising infectivity.

Area of Science:

  • * Molecular biology
  • * Virology
  • * Gene therapy

Background:

  • * Viral vectors offer efficient gene delivery to mammalian cells.
  • * Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile for gene transfer due to broad cell tropism and large capacity.
  • * Targeted gene delivery is crucial for specific applications and overcoming cellular barriers.

Purpose of the Study:

  • * To develop a method for retargeting HSV-1 amplicon vectors to specific cell populations.
  • * To investigate the modification of viral glycoproteins for altered cell binding.
  • * To evaluate the efficiency and infectivity of retargeted vectors.

Main Methods:

  • * Modification of the glycoprotein C (gC) heparan sulfate binding domain (HSBD) in HSV-1 amplicon vectors.

Related Experiment Videos

  • * Replacement of the gC HSBD with a hexameric histidine-tag ligand.
  • * Assessment of vector binding to cells expressing a corresponding pseudo-receptor.
  • * Comparison of modified vector binding and infectivity against wild-type HSV-1.

    • Main Results:

    • * Successful retargeting of HSV-1 amplicon vectors by replacing the gC HSBD.
    • * Modified vectors demonstrated increased binding to cells expressing the specific histidine-tag receptor.
    • * Binding levels to receptor-positive cells exceeded those of wild-type virus.
    • * No loss of infectivity was observed in the retargeted vectors.

    Conclusions:

    • * Retargeting HSV-1 amplicon vectors via glycoprotein modification is feasible.
    • * This strategy enhances specific cell targeting and gene delivery efficiency.
    • * Modified vectors offer a valuable tool for precise gene transfer applications.